Vitrification o embryos i te kawe takitahi

I roto i tēnei tuhinga ka kōrero tatou e pā ana ki te ariā pērā i vitrification o embryos. Dr Kuwayama Masashige i whakaaro tenei tikanga ki te kriotopah ara i roto i te tau te tau. Ko te tamaiti tuatahi whanau i roto i embryos vitrification i roto i 2003. I nui haere oocyte auau ora e 98 ōrau.

Hawhe o nga wahine e farii i IVF, noho nga embryos. puritia hoki ratou cryopreservation e taea tūroro ki te whakaora moni. Whakakore I muri i te katoa, ka whakawhiti i te embryos he nui māmā atu ki te kawe i roto i te tukanga kikiritanga vitro ano. Ko hoki te reira i te ahua o te inihua i roto i te take, ki te kahore he hapu te wahine. Cryopreservation he painga motuhake - ārai i te mate o embryos taea noho i muri i te kawa i.

ontogeny

Hoki te whanaketanga tuakiri o te rauropi, ontogeny ranei, rā te reira hoki i te wa o te kikiritanga me mutu ki tona matenga. he tonu i roto i te wa tenei motini, me te kua he pūāhua pumau. A matou e taea kore ki tona whakatārewa pōturi iho tona whanaketanga ranei. Ko fakanatula reira he okotahi. Ko nga otaota, hātaretare me tae noa etahi vertebrates timatanga e i iti pāmahana, e kore e whakaatu i ngā āhuatanga motuhake o ngā kaiao ora enei.

He aha te mea te moetanga?

Vitrification o embryos kōrero i raro nei. ka karanga wā takitahi o te marino te moetanga. Hei tauira, he maha kararehe ora nga pāmahana Siberian pa -90 nekehanga, me te maroketanga tata oti. I roto i te ako o tenei wā o ontogenesis i roto i te natura i reira ko te pātai e pā ana ki te whakamahi taea o pāmahana iti mo te arumanga wāhanga, me te reversible o te mahi o nga mea whai tuaiwi teitei, me te tangata.

cryopreservation

Cryopreservation - Ko te tikanga whai hua mo te whakatārewatanga o ngā tukanga koiora i roto i ngā pūtau i te rongo ki iti pāmahana. Tenei tiakina ana te mahi ora o ngā pūtau i roto i te whakawera. Na roto i te rongonui, tenei tikanga he vitrification kukune iho. 1 kriotop (krionositel tapanga) kei i 1 ki te 3 embryos.

Hei tauira, ka mahi ana i te tukanga pērā i IVF, whakaaro te pānga pai te ki kia neke i roto i te kōhao uterine kore te mea neke atu i te rua embryos. Ka taea te cryopreserved te embryos kounga toe mō te whakamahi heke mai. Ka taea e ratou hoki te tono mō te re-IVF i muri i te ia, ki te e whakaatu i te tukanga he hua kino. Ki te kawea taua whāinga embryos vitrification i runga i kawe takitahi.

I roto i ētahi wā, whakatio embryos katoa. No te wahine e whai syndrome hyperstimulation ovarian whakamana i roto i superovulation arataki te tino maha. Wai i tūtohutia mīti? Tūroro mate ana i te mate pukupuku, i roto i ngā i mua i te hahau tukanga iraruke ranei. Kei te ka te raraunga whakawhiti embryos ki te kōhao uterine. Whakatio whakaatu tangata katoa e te tupono o hapūtanga i muri IVF mo etahi take tuku iho. kia polyps endometrial, iti matotoru endometrial ki te wa whakawhiti whakaritea, toto aueue tenei.

wāhanga mīti

I whakatotoka ana te embryos i ngā wāhanga:

• hua wairakau (kākano);
• kuru embryos atamira;
• blastocysts.

I te kau, i reira e rua ngā tikanga o te mīti embryos.

mīti puhoi

kawea Vitrification o embryos i roto i te mīti puhoi. whakaarohia he tikanga taua i roto i nga 70, me tetahi o nga tikanga puāwaitanga tuatahi o mīti embryos. Kei te hāngai te reira i runga i tautauā puhoi i te tere aumou. Kia e penapena te embryos i roto i te hauota wai.

Ko Me kï reira e i mīti puhoi i roto i te otinga cryoprotective e hanga e tioata mōkitokito e pēhia ngā te pūtau o te ira. Ka taea e meinga te reira i te whakangaromanga wāhanga katoa ranei o rauemi koiora ki runga ki whakawera. Ko te maha o embryos pai whakawhitia i roto i te mīti puhoi me te hukapapa,, tata 70 ōrau.

vitrification

I muri i 2010, ka anga ki te tono i tētahi tikanga hou, me te ake whai hua o te cryopreservation - vitrification. Ina whakaritea ki te tikanga o mua, ko te ara super-nohopuku ki te whakatio rauemi koiora tenei. Nuinga o ngā wā kawea embryos i muri i vitrification PGD (tātaritanga ira).

A, no te whakamahi i tenei tikanga otinga cryoprotective, te wahi e whakanohoia embryos e kore te hanga tioata i roto i te mīti. Ko te kupu, heke outage kukune tūponotanga. Ko te kaupapa matua o tenei tikanga, ko te kore noa he ara o mīti, engari ano hoki te tere ora o embryos i muri i te hukapapa. E ai ki ngā tatauranga, ko te kore e iti iho i 95 ōrau te maha o ora embryos i muri i te tukanga vitrification.

Eaha te tupu i muri i te hukapapa?

I muri hukapapa, i te embryos kore e rerekē i embryos noa. tango rite pai ratou pakiaka, me te tupu. Na roto i te para embryos katoa i raro ki te pao āwhina. I roto i te whakahaere i tenei mahi wehea te kahu mata o te kukune te taiaho kurupae i te koki e hiahiatia ana, me te haumaru. whakahaere tenei te ira i roto o te anga, me te whakanuia ana te taea o te transplantation angitu ki te kōpū.

Mīti embryos taea rokiroki mō te wa roa. Ko-utu whai hua tenei tukanga, no te mea he iti iho i te he tukanga tuarua o roto i vitro kikiritanga te utu o te tiaki, te hukapapa me te tūhāhā te kukune ki te kōpū.

whakaaro Vitrification te rite te whakawhitinga poua te wahi i te otinga matao i raro meangiti i raro i te pāmahana whakawhitinga karaihe. I te wa ano tonu reira hanganga amorphous o te karaihe, me te whiwhi i te kounga rite ki totoka tioata. Ko te kupu, e rua nga pūtau ora, a tae noa te kukune katoa e puta ki "karaihe". whiwhi hanganga waiwai o te wai i raro i vitrification tika ki tona tautauā tere, iti i.e. te piraunga o te wai i roto i te wā poto o te wā atu i tika hanganga te piraunga karaihe.

I roto i te mau parau ohie, e kore e te wai whakahukapapa ki te ki tona huarahi te piraunga te piraunga karaihe. Otiia i roto i te tikanga ki te tika vitrified kaiao ora, he mea e tika ana ki te whakatutuki i te tere o te pāmahana heke o ≈ 108 ° C / min., Ka ko e taea i roto i te mahi, no te mea kahore e ranei ma tenei pāmahana te wai cryogenic tono, a kore e taea te whakamahi vitrifitsiruemy otinga i roto i te rōrahi iti atu i te rōrahi oocyte. kōrero katoa tenei me vitrification o embryos. He aha te mea te reira, he mea inaianei nui atu iti iho ranei mārama.

i taea e ki te whakamatau e te hua i runga i te Wenerei ki whakatio cryoprotectants taea ki a koe hohoro raro i te tere mīti Scientists. No reira, i te kiato o te 10% glycol propylene me tere etilengligolya te nui iti, i 40 ōrau o te kiato o te vitrification he taea i te tere meangiti o 10 ° C / min, me i 60% makaka auau ki te 50 ° C / min. Otiia ki te hua i roto i te mātotoru o cryoprotectants, taka i runga i te Wenerei, te whakanui ake i to ratou pānga tōraro i runga i te mīti o biomaterials. mīti puhoi whakaoho whakaeminga o te wai mātao i roto i te rauropi koiora me te huānga intracellular. Puta tika ki ngā pūtau maroketanga nui ki te ahua o te hukapapa wahopūtau te huru taua.

Fakatatau ki ai, ki runga ki te taenga o ngā hanganga tangiwai whatiia matū me ngā tukanga tinana o te maroketanga. Ahakoa te meka e ko te vitrification o embryos (e ko, kua reira kua whakaahuatia i roto i te taipitopito i runga) tino pakeke pūnaha tinana, Ka taea e rauemi o tenei hanganga e kitea i roto i to tatou ora mahana (karaihe, silicon, a na i runga i).

Vitrification o embryos: arotake

kohikohi i tēnei tikanga urupare pai anake. He whaihua vitrification tukanga. Otiia kua reira he rota o ngā āhuatanga i roto i ngā wāhanga ngā o te whanaketanga i roto i te taiwhanga IVF. Vitrification - e kore te mea he tikanga hou o te cryopreservation o ngā pūtau ora. Ko ia te taahiraa whakamutunga o mīti puhoi. I teie mahana, e taea ki te whai i te tamaiti no te mea o ngā whanaketanga pūtaiao maha wahine.

ngā kitenga

Nā ki te mahi o maha pūtaiao vitrification taea te whakamana i waho te whakamahi o te utu kōpaka programmed me te whakamahi i ngā taputapu ohie e aroturuki i te kaiwhakahaere. Ko te kupu, ko te tikanga māmā me te putanga ake. Ahakoa te moni nui i roto i cryopreservation, te whakatinanatanga o te tiaki tika o tenei ra kaiao ora i iti te pāmahana e taea.