Rāpoi Diagnostics: wā-tūturu PCR

I tēnei wā, hangaia tātaritanga rāpoi tikanga tauhohenga polymerase chain (PCR), i ko te hiringa mo te whanaketanga o te maha nui o ngā tikanga o te tātaritanga o mate, e hāngai ana i runga i te faaotiraa o te rauemi ira. Hoki e mau ana i roto i te wā-tūturu PCR whakamahi kongakonga ira (i roto i ngā te waikawa karihi) o te kaihoko pūtake, e kore e nei i kitea i roto i te tahi atu mau taata.

Ko te parau tumu o PCR ko te whakareatanga o te tetahi miriona tukumate DNA kongakonga o nga wa i te DNA polymerase i roto i te kōwae motuhake, tuku ki rite te cycler waiariki, e whakarato ana te tukurua huringa o te tikanga pāmahana mo te hōtaka i homai.

Tātaritanga PCR taea ki te faahiti i te raupapa o faarahihia, te pupuri i te mahi paru. A, no te te amplification e wahi mania, i roto i tetahi huringa te maha o ngā kape o te ngā wahi o DNA o te tukumate rearua, i muri i toru tekau huringa - te rau me te tekau. Ko whakamahia aha te PCR i roto i te tātaritanga ki te whakatau i tukumate nui iti, te tāututanga o te mea e taea, kahore reira.

Me kï reira e kore e taea e te RNA hei he tauira mo PCR i te wā tūturu. Na reira, i roto i te tikanga ki te kitea RNA viral i mua i te amplification ki te kia ia faatanohia te DNA ki te kongakonga hāngai o reira. Ko te tauhohenga polymerase chain taea ki te whakaputa i te DNA me te RNA ine tukumate. Kei te whakamahia te mōhiohio kupu whiwhi hoki te aroturuki i te whai huatanga o te therapy whakamahia, me te aro mātai o te waitohunga haumanu.

I tua atu, e kore e wā-tūturu PCR rapu atu mahi ki te faatura ki te wetemunatanga o te DNA ūnga amplicons ranei, e mahi rite te arai ki te tātaritanga tika, me te arahi ki hua teka. hanga ana tēnei huarahi e kore e taea te reira ki te whakamahi i te taahiraa electrophoresis, e arata'i ki te hua mōrearea-whakaiti o te tauhohenga polymerase chain. Hoki, e tika ana ki te hekenga i roto i te maha o whawhe ki te kaihoko, te whakaiti i te wa o te rangahau, e ohie te reira i te tukanga, te whakaiti i te tūponotanga ka o ngā hapa. taea hoki PCR ki a koe whakapūtā te maha tuatahi o kape o te DNA o te tukumate i roto i te tamataraa haumanu ki te āwhina i te tāutu i ngā wā o exacerbations me ki te tango i mehua wā ki te whakaora i te manawanui.

I teie mahana, i reira e he maha nui o ngā tikanga amplification o warea i te wā tūturu. Ko te ngā tikanga tino whakamahia nuitia tono amplicon fakafekau'aki ki tirohanga fluorogenic, te kaha radiation o e rerekē i runga i te haaputuputuraa o te amplicon me tuhia i roto i te wā tūturu i te tikanga o te pūrere motuhake i huaina he thermocycler ki te kōwae whatu. E whakarato ana i te taumata tiketike o te tūtohutanga o te tātari. I te hua PCR taua taea te kitea, me ki te rahinga nui o e-hua, i te wa ka whakamahi i kore te electrophoresis reira i roto i ētahi wā ite.

E PCR te whakamahi i ngā tauira mana pai, me te kino i roto i te wā tūturu. Ko te kupu, tātarihia ngā tauira pai me te kino i roto i te ako rite tauira motuhake, he tauira mana whakaurua ai te i roto i ia taua tauira, me te haere kaupae tātari katoa.

I teie mahana, i reira e he maha nui o ngā pūrere mo e mau ana i roto i te tauhohe polymerase chain. Ko ētahi o ēnei Bio-Rad, Applied Biosystems, Cepheid ko Roche. He huapai me ngā huakore ia o ratou

Me kï reira e te PCR - tikanga o te tātaritanga whakamahia i roto i te nui-tauine pūtaiao, rangahau me ngā pokapū tātaritanga huri noa te ao, no te mea homai reira te whai wāhi ki te kawe i roto i tikanga tonu o nga hua.