Hono immunosorbent anga matou: tono me ngā āhuatanga o

Hono anga matou immunosorbent - ako taiwhanga, e hāngai ana nei i runga i te tāutu o complexes "antigen-antibody" ki te tapanga enzymatic motuhake e tautuhi, e te huri i te tae o te tïpako. Ko te whakaaro matua o tenei tātaritanga - faaotiraa o te aroaro o ngā hua ngā o tauhohe enzymatic i roto i te tauira whakamātautau.

Hoki rapunga o antigens me paturopi te whakamahia immunoassay paru heterogeneous, mai te mea ngāwari tona tukanga ki te wehe i te wāhanga o te tauhohenga me ki whakaroa etahi o ratou i roto i te wāhanga mārō.

E tano ia tapao i te painga e whai ake nei o tenei ako taiwhanga:

• tūtohutanga tiketike, e taea kitea ara he maha iti o ngā rāpoi ngota o te matū;

• mō te e mau ana i roto i tenei tātaritanga taea te whakamahi i te maha itinga o te rauemi whakamātautau;

• te taea o te wa roa ki te pupuri i nga wae katoa e kua hiahiatia hoki tātaritanga;

• ki te Easy mahi;

• wātea o ngā mauhaa me ataata;

aunoa • immunosorbent anga matou te i ia wāhanga o te tauhohenga;

• kete utu āhua iti e kua whakamahia mō te tātaritanga.

Ēnei painga kia whānui te whakamahi o IFA i ngā wāhi o te rongoā, tae atu ki, hoki te tātaritanga o wheori.

Ko te tukanga tino noa mō te immobilizing paturopi me antigens ko te tauhohenga adsorption, ina e piri te rāpoi motuhake ki te wā totoka (e tika ana ki taunekeneke katote hydrophobic ranei i hanganga ranei o here hauwai).

Me mea e ahau e whakamahia te immunoassay paru i roto i te wā e rua:

• ki te tautuhi antigens o tukumate o ngā mate;

• ki te whakatau i te aroaro o paturopi ki te antigen o microorganisms pathological (kia tuhia te reira e taea te Elisa kitea tata tetahi mate taihemahema).

I whakamahia pereti terei Motuhake ki te kawe i tenei serodiagnosis. He 96 rua i runga i te taha o te antigen adsorbed. Ki runga ki te urunga i roto i te papa anga, he paturopi antigen pūtau e piri tika. Ko te tua o muri o te antibody tapanga ki te puna o immunoglobulins tangata toro tauhohenga motuhake. I roto i te serum chromogen maimoatanga e whai ake nei (tae) rerekē tïpako tae (i roto i te aroaro o nga complexes hāngai "antigen-antibody"). E tano ia tapao e he tika hautanga ki te nui o paturopi te kaha tae.

Elisa Analysis mutu ine i te kiato whatu o te wai i roto i te poka. Ko te kupu te whakamahi i ngā pūrere motuhake me ngā tauira-whāiti e te tauturu i te tatau i te kukū antibody. Kia mahara te reira e ki nga pūnaha whakamātautau whakahaere kua ona uara o tohu paerewa tenei tātaritanga (tenei te nuinga o te wā whakaritea i roto i nga hua, e kua hoatu i roto i).

Mā te Elisa tātari pakewakewa, kakā viral ko HIV, me te kitea i roto i te cytomegalovirus tangata tinana, Chlamydia, herpes (momo rerekē). āwhina hoki tēnei tātaritanga ki te whakatau i te taumata o hormones ki te kitea tohu mate pukupuku.

Hea ki te kia whakamatauria i Moscow? I teie mahana, i reira e he rota o taiwhanga motuhake, e whakaherea he whānui o ngā whakamātautau serological. Hoki tohutohu kōrero tēnā he tākuta o te whare hauora rohe (tūmataiti ngaio ranei, dveryaete koe). Ki te hua whakamātautau kia haere hoki ki te tākuta. Na whakarongo ki ana kōrero i runga i te hiahia mō tetahi maimoatanga.