TLC tikanga ohie tātaritanga matatini

TLC Ko te rangatira i roto i ngā take o te tātari ine, me te āhua-ine o rongoā matatini, māori, biomedical, hangarau, matū, me te tokomaha atu matū. Ko hoki tūrara kahupapa te tikanga tino wātea o te tātari papatipu o tata akomanga katoa o matū.

Kei te whānui tenei tikanga o te wehenga me te tātaritanga o ngā pūnaha maha-wae whakamahia i roto i te taiwhanga o te pū rawa rerekē, me te aronga: parukore-tahumaero, kararehe, agrochemical, rangahau, whare whakawā, me hoki teihana tiaki tipu, Center mo Waeine me te Bureau o Forensic Medicine.

Ko te painga matua o kikokore-apa tūrara, e hāngai ana i runga i nekehanga eluent (analyte whakarewa) i roto i te apa sorbent roto i te ope capillary pera ka karanga ko te tapatahi, me te ohie o te tukanga chromatographic, te tohu tiketike o pū o te tātari me te utu ngā iti o te taputapu e tika ana.

TLC, he nui rawa o te tūtohutanga (raro rapunga paepae) me selectivity, taea ki te whakatau 10-20 matū UG ake ki te 7%, i te mea tiketike rawa. Ka taea e tūrara kahu angiangi kia adsorption me tohatoha momo. Ko te tino whānui whakamahia ko te kōwhiringa tuatahi.

Ko te ngako o te tikanga e takoto i roto i te TLC taonga whakarewa nuku i roto i te moenga sorbent me te tohatoha mokowā o te ngā wae rite ki o ratou āhuatanga takitahi me ngā āhuatanga. Ko te take i te rāpoi ngota o te hanganga rerekē, me te hanganga o te adsorbed (whakatakotoria) i runga i te mata o te pereti chromatographic totoka rerekē. Na reira, kei te āta wehea matatini pūnaha-wae maha ki wāhanga takitahi.

Te neke i kīia e rerekē haere te moenga sorbent, kua wehea te takitahi, te wāhanga i whai ahua ai te chromatogram. Ka hāngai ia wahi tae ki te matū motuhake. taonga taekore ki te marama hihi katinga i raro i te mana o e he plurality o pūhui timata ki te fluoresce, i raro ki te kaiwhakahohe maimoatanga tae motuhake ranei.

I roto i hou tikanga tātari tu GLC i roto. āhuatanga i tēnei tikanga e wehenga me te tātari mā te whakamahi i, rite te kaihoko sorbing motuhake nonvolatile wai, nana ai te te rauemi uku te paura. A, kia rite ki te reo peia whakamahia konei rerekē hau e mangere. he whai hua te nuinga mo te ako o ranunga matatini e roto i rau o wāhanga rerekē tēnei tikanga.

Tētahi atu hou tikanga mō te tirotiro i te hanganga o ngā matū matatini he tūrara wai. Ko te āhuatanga tino o tenei tikanga ngā i roto i te wehenga o ngā wāhanga takitahi mā te whakamahi i te rerekētanga i roto i to ratou tohatoha i waenganui i turanga (eluent) ka whakaritea (adsorbent) wāhanga. Kei te tino maha whakamahia i tēnei tikanga i roto i te tātari o ngā pūhui matū waro (tāpiritanga kai, patukīrea, ngā tāoke, me ētahi atu). Ano, whakamahia he taua tikanga chromatographic te ki ako i te mana kounga o ngā hua rongoā, te faaotiraa o steroids i roto i fluids koiora, me ētahi atu tātaritanga waikawa amino.