Reo Ploskireva (F Bacto-piarimu): whakaahuatanga. koiora Medical

Ploskireva reo (ka huaina i tonu Bacto piarimu F) Ko te reo matūkai mō te maara o etahi microorganisms, matua Shigella ko Salmonella. Ka rite ki te pūtake mō te whakamahi rauemi huaketo ia: mimi, ate, feces.

kōrero

Nikolay Ivanovich Ploskirev - kaikoiora moroiti Soviet me te tākuta Honoured. I whanau ia i te tau 1869, ka mate i roto i 1948, ka mahi te nuinga o tona ora i roto i tona ake whenua - Tomsk.

I roto i te 1888, ia, te pūtaiao heke mai, i waho whakaoti i te kōeke tuaono o te kura, piri te ope. i taea e ki te whakaoti i te whakangungu anake i roto i te 1892 ia, ka haere nga whakamātautau mō te whakauru ki te Imperial Tomsk University, ako ia i te Faculty o Medicine. I roto i te 1898 riro ia te taitara o te tākuta.

whai wāhi Ploskirev i Russian-Japanese War, a muri mahi reira i roto i te fare ma'i Tomsk. I te tau 1910 i whakaritea e ia ki te pou o te matenga o te Tomsk hōhipera Dermatological me paipai, me kua hoki e toru tekau nga tau i tona matenga tūturu. Ko te taua pūtaiao ko tetahi o nga kaiwhakatū o STI whare hauora i roto i Tomsk.

tuhituhi Nikolay Ivanovich Ploskirev te raupapa o mahi i oti rawa ki te whawhai ki mate paipai.

hanganga

Wenerei Ploskireva - rauemi mo te maara o te huakita intestinal me, reira, me i roto i te maha o ngā momo o ngā matūkai. Kei te whakaputaina te reira i roto i te puka maroke.

Tino he ōwehenga nui o tona taimaha katoa he sprat hydrolyzate pancreatic (10.4 g / l). Paku iti kiia hoki te konutai dibasic hydrocitrate (8.5 g / l). Hoki i reira ko te matūkai hupa me te waiu te huka (8.62 me 7.3 g / l) maroke.

Ko te taitara tuarua Ploskireva reo - J. Bacto-piarimu i roto i te hanganga he reira hakari i te nui o piarimu, 6,94 g / l. Seriovatistokislogo ihirangi konutai anhydrous rite ki te 5.1 g / l. He ko te aroaro o te Phosphate anhydrous disodium - 2.1 g / l, tai konutai o waikawa cholic e pā ana ki 3.46 g / l, me te houra pungarehu - 2.4 g

roto Iti iho i te he karamu reira tohu - whero kūpapa (0.05) me matomato ngingila (0.0002). Ko te ihirangi konutawa - 0,13.

tono

Ko te ako o te tukanga matüora o huakita whai wāhi tino nui i roto i te tātaritanga o mate e meinga kaiao hāorakore. Momo i roto i tenei hapu - rau. Ko ratou tata ngā i roto i te Morphology me ngā āhuatanga ahurea, me te ara tino pono ki te wehewehe ratou i ia atu - ko te ako o te matūora.

tīmata te ako ki te Typhoid o rauemi pathological ki te reo ahurea. Ia pārōnaki pathological mō huakita coliform me te wahi o reira, i roto i te tua ki te piarimu mania, ngā marama me te reihuka. Tukatuka kaha o te reihuka - he tohu nui o te pārōnaki o enterobacteria. Ki te kitea te tenei, hünuku te pH ki te kawatanga me whakahohe te tohu e, e tahuri te koroni.

kia whakamahi i ētahi atu whenua atu pāpāho matūkai, engari hāngai ratou tikanga o mahi ki te toru tino noa i roto i Russia - tenei taiao Endo me Levine Ploskireva Wenerei.

Whakaahuatanga o te tikanga

Abroad reo Ploskireva tairitenga he pera-ka karanga te Macconkey Hakara. I roto i te oti puka otinga mārama he tae māwhero-kōwhai te marama. Koroni taea ki te tukatuka reihuka, i roto i Ploskireva hoatu whero (karani) tae. Ki te kore e taea e te huakita tukatuka reira, ranei he taekore koroni ranei he tae ngoikore.

Hoatu e te hanganga o te reo ngā matū inhibitors Ploskireva (matomato ngingila, tai ate, iodine), ki te mea tata rawa inhibits te tupu o otaota karamu-pai, kia rite ki i te eshehiry ra tuatahi tino te tairoiro e te tupu, me te tahi atu microflora te rere mua.

waewae tuarua

Atu o te moni ko te kōwhiringa o te koroni, a ka kua Matete ki runga ki te reo rokiroki tuatahi, me te pārōnaki. Taiao i roto i nei i reira te matomato kia roto i substrates maha. kia kitea te ahurea kitakita mahi enzymatic ki te faatura ki a ratou, ki te reo taua whakanohoia i roto i te ngongo kia reira e rua ngā pae:

• tetahi i roto i nei te piarimu te wakapū;
• ki te tīwae.

Ko te moni o kairangahau i roto i te koroni kapi wahi bevelled patunga mātotoru me te i roto i te tīwae - he hukihuki. I tenei wā, whakamahia te reo Russell, Kligler Olkenitskogo ranei. Ka rite ki pārōnaki tātaritanga whakamahia me taiao Ploskireva.

Microbiology me pathogenic kaiao

Hakara Ploskireva Ko te take nui i roto i te kimi mate meinga e Enterobacteriaceae. Na, mo te tauira, kei te tupu te reira, ka rerekëtanga koroni o microorganisms e meinga koripi bacillary. Tenei kaiao anaerobic no ki te puninga Schigella.

Ka rite ki nga tangata katoa e he wahi o te hapu o huakita intestinal, shigella i te ahua o rakau, te rahi o rua micrometers. e kore e ratou riro capsules me pua i flagella - taea reira ki te kia whakanuia ratou i Salmonella, e, i roto i te tahuri, he pūkoro. Ratou tupu pai i runga i te nuinga o pāpāho ohie, i te pāmahana paku ki runga ake ambient (35-37 ° C) me te 7,4 pH. Na roto i te āhua o te tupu, e kore e koroni rerekē i Salmonella.

Ka rite ki kōrero i runga, ka taea e microorganisms kia rerekētanga nui morphological, Heoi, rerekē tino i roto i ngā tukanga matüora o te ora. Ko te kupu, ki te kahore hoki Shigella Nyukestl, i waho te hanganga o te hau waikawa puta i roto i FERMENTATION o te warowaihā. Ki te okotahi o Shigella sonnei, e kore e taea e ratou rīriri reihuka, engari kua digested kūhuka. Just ki o ratou āhuatanga matua, ka taea e koe te kawe ia ratou ki ta taea ratou ki te whakaiti i niraty ki nitrite. i digested ratou koroni ki Urea, me rite i roto i te reo te ahurea ko kahore aro o gelatin.

Collection, me te faaineineraa o te purapura

Hei kitea āpiha o te koripi, kua hiahiatia whakamātautau microbiological muia taiao, arā, tütae manawanui. Ki runga ki te taenga o te rauemi, e hiahia ana koe rite hohoro rite taea ki te mahi i te ruinga. Ki te kore e taea tenei, me kia whakanohoia te puna i roto i te rokiroki - ranunga moka Phosphate nonireka ranei. I 4 ° C, e taea te rongoa ratou mo neke atu i te ra. Kohikohi me kawea te rauemi i roto i na tikanga o te ngongo karāhe rectal e whakaurua nei ki roto ki te tōu.

No te rangahau o te rauemi tīpakohia mongamonga purulent hūpē ki kia horoia i roto i rua ki te toru korere konutai isotonic otinga pūhaumāota.

Taupānga o huakita pathogenic i roto i te taiao Ploskireva hanga rapa karaihe. Me koe ki te mirimiri i runga i te wahi iti o tona piarimu, ka haea atu te rapa i runga i te taiao, me te pania te rauemi wäriu ki te mata whakatokia. Ki te mahia te matomato i roto i te torutoru kapu, ka hiahia ki te whakato i te puranga hou ki ia o ratou.

Ki te mongamonga muco-purulent reira i roto i te tango i runga i te tātaritanga i whakatapua, e kore te auraa i te reira e te mea kahore he aroaro o tukumate. I roto i te take i taua he mea e tika ana ki te whakaehu feces i te 10 ml o te konutai pūhaumāota (kukū 0.85%) me ka rui kotahi e rua ranei pata i runga i te reo Ploskireva. Ka taea te whakatokia heamana Non-emulsified i roto i te hupa selenitovy. whakamahia ia ano, ki te, hei utu o nga feces he ruaki horoinga ranei.

tātaritanga microbiological

I roto i kawea te wāhanga tuatahi o rangahau microbiological i roto i purapura o huakita pathogenic i roto i rua kapu, ka ki mataara pehea ki te tupu Shigella. I te Wenerei hua Ploskireva he hua, me te tuarua - i runga i te taiao Endo Levin ranei.

Nā ki te meka e reira nga taumahatanga o te shigella e ātete ki paturopi, ki tāpiri te reo i ki chloramphenicol. Na ka puta te reira i roto i nga ra e heki i roto i te incubator i 37 ° C.

I te rua o nga ra ki te tūhura i te koroni tupu. Te hunga e kore e ki runga ki te reo tātaritanga pārōnaki tupu te huitahi e, ko te reira e tika ana ki te tarutaru i roto i reo poto ranei o Russell "rarangi paipōro." Kei te whakahaere rangahau anō i te hātepe o te hua tuatahi ki runga ki a ratou. Koroni o Shigella i runga i te Wenerei Ploskireva tupu i roto i te puka o tīwae mārama, taekore me te iti. Ko ratou o rua momo:

• papatahi ki tapa mākini;
• porotaka, ohu nei, ki te whiti mākū angamaheni.

Me mikroskopirovat E toru e wha ranei koroni, a te uiui hoki te nekeneke. Ki te kore e te whakamutunga whakaatu ake, nekehia ratou i runga i te Wenerei Olkenitskogo ki tutakina i te ahurea parakore. I roto i te ngaro o koroni āhuatanga Shigella ranei, ki te kitea kahore he tupu te i te katoa, he mea e tika ana ki Endo piarimu Ploskireva hua matomato o te hupa selenitovogo ranei. Ki te he hakari i rawaka tau te koroni angamaheni, hoatu te agglutination tata. He ranunga o te sera sonnei me Flexner, puta ai te tauhohenga te karaihe.

rangahau atu

I te toru o nga ra i reira he huringa e kua puta i roto i te koroni i runga i te reo Russell o. Ki te kahore he he tikanga e kore e e haafaufaa i te reihuka me ferments kūhuka i ki te whakaputa i te waikawa, kua wehea taua mea, ka tātarihia, whakapau Microscopy, me te kawe i rui i roto i te "rarangi paipōro," engari tenei wa whakawhānui. Just hoatu te tauhohenga agglutination, i roto i te tikanga ki te tāutu serological.

I te ra whakamutunga, e taea te mutu te reira i runga i te pūtake o ngā rerekētanga i roto i te "tau paipōro" (ki te reira he FERMENTATION o te warowaihā), me te whakarāpopoto i te tauhohe agglutination.

Rokiroki me te faaineineraa

Te faaineineraa o piarimu Ploskireva puta e whai ake:

1. whakaohokia taonga maroke 55 g te i roto i te rita o te wai māturu.
2. Kohuatia hoki e rua ki te toru meneti noa e kore e ano tino rewa te piarimu.
3. Ringihia ki rihi Petri (sterility kōwhiri) paparanga 5-6 mm o.
4. Kua mahue tuwhera nga papa paraharaha, mo te hawhe te haora i te pāmahana rūma (18-25 nekehanga). I muri i tenei wa te reo, ko te makona whakapakeketia me maroke.

Wenerei Ploskireva photosensitive me hygroscopic. kia penapena i te puehu i roto i te mōkihi hiri, kahore me nui te haumākū whanaunga i roto i te ruma 60%. Ko te pāmahana tino arotau mō te rokiroki o te 2 ° C ki te 25 ° C. Me ū rokiroki ki enei ture, te kore taea kitenga hē.